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EBS users can update planting protocols for either individual or multiple occurrences in Experiment Manager. Let’s get started on updating them.

Update individual protocol

You may update the existing protocol from one of your occurrences. Take note that the protocols listed depend on the experiment type.

1. In the experiment manager table, go to the row of your selected occurrence. On the left side of the row, click the Edit Experiment and Occurrence () button.

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2. Click on the Protocols tab. You can now specify the (A) planting, (B) traits, (C) management, and (D) harvest protocols.

Set the planting protocols

1. Under planting instructions, you can specify the crop establishment, planting type, and plot type.

2. After selecting the plot type, indicate the required information marked with an asterisk ( * ). You may also write planting instructions in the text field. This is optional.

3. Once done, click Save. Click on the next protocol tab on the left side.

Set the pollination instructions

Intentional Crossing Nursery, Cross Parent Nursery, Generation Nursery

1. Click the Pollination subtab. Provide pollination instructions for your experiment in the text field provided. You are encouraged to do this step.

2. Once done, click Save.

Set the trait protocols

You may provide the traits one by one or select existing trait protocols (which are a group of selected traits previously used in an existing experiment).

Select individual traits

1. Click the Traits subtab.

2. Under the Trait drop-down field, select the trait you want to add.

3. Click Add to move it to the Selected Traits section.

4. Repeat steps 2 and 3 until you are satisfied with your selected traits. Click on the next protocol tab on the left side.

Select trait lists

1. From the Traits subtab, click the Trait Lists subtab.

2. Under the Trait List drop-down field, select the trait protocol that you want to add.

3. Click Add to move all the traits to the Selected Traits section.

4. You may add instructions by ticking the checkboxes beside the selected traits and clicking the Bulk update button ().

5. Provide the instructions you want to indicate and click All or Selected (if you have selected traits).

6. You may also delete any selected traits by ticking the checkboxes beside them and clicking the trash button .

7. Your changes are automatically saved. Click on the next protocol tab on the left side.

Set the management protocols

You can specify the management attributes to be observed one by one or select existing management protocols (which are a group of selected management attributes previously used in an existing experiment).

Select individual management attributes

1. Click the Management Variables subtab.

2. Under the Management Attribute drop-down menu, select the attribute you want to add.

3. Click Add to move it to the Selected management attributes section.

4. Repeat steps 2 and 3 until you are satisfied with your selected management attributes. Click on the next protocol tab on the left side.

Select management variables lists

1. Click the Management Variable Lists subtab.

2. Under the Management Variable List drop-down field, select the list that you want to add.

3. Click Add to move all the attributes to the Selected management attributes section.

4. You may add instructions to the management attributes by ticking the checkboxes beside them and clicking the Bulk update button ().

5. Provide the instructions you want to indicate and click All or Selected (if you have selected management attributes).

6. You may also delete any selected attributes by ticking the checkboxes beside them and clicking the trash button .

7. Your changes are automatically saved. Click on the next protocol tab on the left side.

Set the harvest protocols

1. Specify the method of harvest by clicking on the drop-down field under Harvest Method.

2. You may also add additional harvest instructions in the text field below.

3. Once you’re satisfied, click Save.

4. When the changes have been applied, click ← on your browser (or click the Experiment Manager heading) until you are back at the Experiment Manager table.

Update occurrence protocols

You may group multiple occurrences and update the existing protocols.

1. In the Experiment Manager table, click the of the occurrences. Then, click the Update experiment- or occurrence-level data () button > Update occurrence protocols.

2. You will be directed to the Protocols browser with the list of the selected occurrences. Click the drop-down tab to display the occurrences

3. Set the protocols as needed. Refer to the section above for the steps in updating protocols.

Great job! You have updated the protocols for your occurrences. You can also explore other tools in Experiment Manager that will be helpful in your experiments.