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How to request for F1 hybridity test?

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To check if the F1 genotype matches the expected genotype, F1 hybridity testing is done. As such, users can now generate F1 hybridity results using genotyping requests with the purpose of Hybridity Testing in the MDA tool. Take note that all genotyping data found on the MDA data browser are sourced from the Request manager tool.

Request for F1 hybridity test

Once logged in to the Breeding Analytics domain, open the (blue star) left navigation menu and select the Molecular Data Analysis tool.

Contact a data manager or send a support ticket if log in problems are encountered.

  1. Open a genotyping dataset with the purpose “Hybridity Testing” by clicking the (blue star) view icon.

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  1. Click the Request Analysis button on the upper right corner of the screen.

If the Request Analysis button is not available, it is because there are no genotype data displayed on the Genotype Data tab data browser. Adjust the default call rate thresholds to display the excluded data.

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View and download results for F1 hybridity test

As soon as the request is placed, analysis results are immediately available for viewing.

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  1. The F1 Hybridity Results data browser shows a result of either TRUE or FALSE will be shown, where:

  • TRUE indicates that the F1 genotype and the expected genotype are a 100% match, while

  • FALSE indicates that the F1 genotype and the expected genotype are not a 100% match.

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  1. On the toolbar, provide a preferred file name on the text field or use the auto-generated one. Click the (blue star) Download icon to start the download process.

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  1. A CSV file is exported containing the results.

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Great! You have successfully requested an F1 hybridity test for your genotype data!

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